Quantitative Analysis of Peroxisomal Protein Import in Vitro
نویسندگان
چکیده
منابع مشابه
Quantitative analysis of peroxisomal protein import in vitro.
Protein import into the peroxisome matrix is mediated by peroxisome-targeting signals (PTSs). We have developed a novel, quantitative, in vitro assay for measuring peroxisomal import of PTS1-containing proteins. This enzyme-linked immunosorbent assay-based system utilizes semi-intact human A431 cells or fibroblasts and a biotinylated version of the PTS1-containing import substrate, luciferase. ...
متن کاملRecent advances in peroxisomal matrix protein import.
Peroxisomes are essential organelles responsible for many metabolic reactions, such as the oxidation of very long chain and branched fatty acids, D-amino acids and polyamines, as well as the production and turnover of hydrogen peroxide. They comprise a class of organelles called microbodies, including glycosomes, glyoxysomes and Woronin bodies. Dysfunction of human peroxisomes causes severe and...
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oxisomal matrix proteins are imported into the organelle from their site of synthesis on free cytoplasmic ribo-somes. This observation led to the classical model of peroxisome proliferation in which existing peroxisomes Amherst, Massachusetts 01003 increase in size via the direct import of proteins from the cytoplasm, and divide by fission or budding (La-zarow and Fujiki, 1985). As such, the bi...
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Cytosol-dependent peroxisomal protein import in a permeabilized cell system
Using streptolysin-O (SLO) we have developed a permeabilized cell system retaining the competence to import proteins into peroxisomes. We used luciferase and albumin conjugated with a peptide ending in the peroxisomal targeting sequence, SKL, to monitor the import of proteins into peroxisomes. After incubation with SLO-permeabilized cells, these exogenous proteins accumulated within catalase-co...
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ژورنال
عنوان ژورنال: Experimental Cell Research
سال: 2001
ISSN: 0014-4827
DOI: 10.1006/excr.2000.5111